PDGF Activates K-Cl Cotransport Through Phosphoinositide 3-Kinase and Protein Phosphatase-1 in Primary Cultures of Vascular Smooth Muscle Cells
Document Type
Article
Publication Date
7-15-2005
Abstract
K-Cl cotransport (K-Cl COT, KCC) is an electroneutrally coupled movement of K and Cl present in most cells. In this work, we studied the pathways of regulation of K-Cl COT by platelet-derived growth factor (PDGF) in primary cultures of vascular smooth muscle cells (VSMCs). Wortmannin and LY 294002 blocked the PDGF-induced K-Cl COT activation, indicating that the phosphoinositide 3-kinase (PI 3-K) pathway is involved. However, PD 98059 had no effect on K-Cl COT activation by PDGF, suggesting that the mitogen-activated protein kinase pathway is not involved under the experimental conditions tested. Involvement of phosphatases was also examined. Sodium orthovanadate, cyclosporin A and okadaic acid had no effect on PDGF-stimulated K-Cl COT. Calyculin A blocked the PDGF-stimulated K-Cl COT by 60%, suggesting that protein phosphatase-1 (PP-1) is a mediator in the PDGF signaling pathway/s. In conclusion, our results indicate that the PDGF-mediated pathways of K-Cl COT regulation involve the signaling molecules PI 3-K and PP-1.
Repository Citation
Zhang, J.,
Lauf, P. K.,
& Adragna, N. C.
(2005). PDGF Activates K-Cl Cotransport Through Phosphoinositide 3-Kinase and Protein Phosphatase-1 in Primary Cultures of Vascular Smooth Muscle Cells. Life Sciences, 77 (9), 953-965.
https://corescholar.libraries.wright.edu/ptox/218
DOI
10.1016/j.lfs.2004.08.046
