Interaction of Sodium Nitroprusside/Liposome System With Bovine Serum Albumin: A Short-Cut Determination
Document Type
Article
Publication Date
9-1-2003
Abstract
A rapid determination of protein-liposome binding was developed to predict the circulation time of the system within an animal, which is a function of the amount and type of protein bound. The binding pattern of albumin to liposomes, with and without sodium nitroprusside (SNP), was analyzed by SDS-PAGE. Liposomes were made of egg yolk lecithin, soybean lecithin and dimyristoyl lecithin, and contained SNP. They bound 58%, 26% and 100% bovine serum albumin, respectively, when compared to their corresponding controls lacking SNP. The method applied is simpler and significantly faster than ordinary chemical determinations.
Repository Citation
Taira, M. C.,
Stern, A. L.,
Escobar, M. E.,
Adragna, N.,
& Alonso-Romanowski, S.
(2003). Interaction of Sodium Nitroprusside/Liposome System With Bovine Serum Albumin: A Short-Cut Determination. Biotechnology Letters, 25 (17), 1425-1429.
https://corescholar.libraries.wright.edu/ptox/225
DOI
10.1023/A:1025051511608
