Effect of O-Phenoxy Caspase Inhibitors on Apoptosis

Authors

Benjamin K. Southerland
William C. Grunwald Jr., Wright State University - Main CampusFollow
Kashmira Kulkarni-Datar
Chanel Hagler
Daniel M. Ketcha, Wright State University - Main CampusFollow
Thomas L. Brown, Wright State University - Main CampusFollow
David R. Cool, Wright State University - Main CampusFollow

Document Type

Presentation

Publication Date

4-2008

Abstract

Apoptosis is a process of cell death, in which a cell commits suicide. Apoptosis is essential for the maintenance of homeostasis and apoptotic dysregulation has been implicated numerous human diseases. Apoptotic events are mediated through a unique family of aspartyl proteases, caspases. Our study tested the effectiveness of nontoxic, O-phenoxy-conjugates caspase inhibitors to prevent cell death. Human leukemia cells were treated with Boc-D(OMe)-OPh, Cbz-D(OMe)-OPh, Q-D(OMe)-OPh, Boc-VD(OMe)-OPh, Cbz-VD(OMe)-OPh, Q-VD(OMe)-OPh, and Q-VE(OMe)-OPh. The cells were induced to undergo apoptosis and the inhibitory effectiveness of the compounds was evaluated. Our results indicate the drugs that contained the dipeptide VD were more effective at inhibiting cell death than those that contained aspartic acid (D) alone. Q-VE(OMe)-OPh had no apoptotic inhibitory activity and was identified as the first true O-phenoxy conjugate, negative control. Our data suggest that N-terminal protecting groups and amino acid composition significantly alter the effectiveness of peptide-based cell death inhibitors.

Comments

Poster presented at the 235th American Chemical Society (ACS) National Meeting, New Orleans, LA.

Repository Citation

Southerland, B. K., Grunwald, W. C., Kulkarni-Datar, K., Hagler, C., Ketcha, D. M., Brown, T. L., & Cool, D. R. (2008). Effect of O-Phenoxy Caspase Inhibitors on Apoptosis. Abstracts of Papers - American Chemical Society, 235.
https://corescholar.libraries.wright.edu/ptox/32