Publication Date
2015
Document Type
Thesis
Committee Members
Nancy Bigley (Advisor), Cheryl Conley (Committee Member), Barbara Hull (Committee Member)
Degree Name
Master of Science (MS)
Abstract
Flow cytometry is the standard method used to diagnose, stage, and monitor patients' response to the treatment given by counting the numbers of CD5, CD19 and CD5+ CD19+ B lymphocytes. In this study, a comparison was done between numbers of single CD5+, single CD19+ and dual CD5+ CD19+ mouse spleen B lymphocytes using flow cytometry and antibody-latex beads. The bead method involved antibody-coated latex bead and yielded results similar to those of flow cytometry. For cells exhibiting both markers (CD5+ CD19+), the bead method used antibody-coated beads of two different colors yielded similar results to those of flow cytometry results. These findings show that the antibody-coated beads are adequate to determine the numbers of single CD5+, CD19+positive cells and CD5+ CD 19+double positive B lymphocytes under circumstance where flow cytometry is not available.
Page Count
63
Department or Program
Microbiology and Immunology
Year Degree Awarded
2015
Copyright
Copyright 2015, some rights reserved. My ETD may be copied and distributed only for non-commercial purposes and may not be modified. All use must give me credit as the original author.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
