Publication Date
2017
Document Type
Thesis
Committee Members
Thomas Brown (Committee Member), Adrian Corbett (Committee Member), Debra Mayes (Advisor)
Degree Name
Master of Science (MS)
Abstract
We used the chicken embryo model to examine whether altered incubation temperature during chicken embryo development would alter the protein expression of heat shock protein 70 (hsp70), hsp90, and connexin 43 (cx43) in the brain. We incubated eggs in either optimal (37.6° C, Con), increased (39.6° C, +2), or decreased (35.6° C, -2) temperature conditions until they reached embryonic day 16 (E16). The -2 condition significantly increased incidence of embryonic mortality. As shown in the literature, the development of the eggs was accelerated or decelerated due to increased or decreased temperature respectively. To control for this, we incubated eggs at optimal temperature (37.6°C) to the developmental stage of the +2 (E17.5, HH43-44) and -2 (E12, HH38) conditions. Embryonic chicken brain protein was quantified for hsp70, hsp90, and cx43 using Western Blot analysis. We found that the +2 condition significantly increased cx43 and hsp70 protein expression, but had no effect on hsp90 protein expression.
Page Count
47
Department or Program
Department of Neuroscience, Cell Biology, and Physiology
Year Degree Awarded
2017
Copyright
Copyright 2017, all rights reserved. My ETD will be available under the "Fair Use" terms of copyright law.
