Publication Date
2019
Document Type
Thesis
Committee Members
Ji C. Bihl (Advisor), Adrian M. Corbett (Committee Member), Yanfang Chen (Committee Member)
Degree Name
Master of Science (MS)
Abstract
In this study, we tested the protective effects of EPC-EXs (endothelial progenitor cell derived exosomes) and miR-126 EPC-EXs (microRNA-126 EPC-EXs) on the astrocytes injured by HG (high glucose) plus H/R (hypoxia/reoxygenation) model. At first, we determined the concentration and time dependent uptake of EPC-EXs by astrocytes. It was also found that the EPC-EXs were uptaken via macropinocytosis, caveolin-dependent and clathrin-mediated pathways in astrocytes. Furthermore, the astrocyte cell line was injured through the HG + H/R model. EXs, isolated by ultracentrifugation from the EPC culture supernatant were co-incubated with the injured cells. It was found that EPC-EXs and miR-126 EPC-EXs decrease apoptosis, lipid peroxidation, oxidative stress and cytotoxicity in the injured cells, hence protecting them.
Page Count
80
Department or Program
Department of Pharmacology and Toxicology
Year Degree Awarded
2019
Copyright
Copyright 2019, some rights reserved. My ETD may be copied and distributed only for non-commercial purposes and may not be modified. All use must give me credit as the original author.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
ORCID ID
0000-0003-0936-6925
