Publication Date
2020
Document Type
Dissertation
Committee Members
Yanfang Chen, M.D., Ph.D. (Advisor); Ji C. Bihl, M.D., Ph.D. (Committee Member); Ravi P. Sahu, Ph.D. (Committee Member)
Degree Name
Doctor of Philosophy (PhD)
Abstract
In this study, we tested the protective effect of UV-B treated HG-HaCaT-Exos on Schwann cells (SCs) injured by HG (high glucose) model. At first, we determined the dose of UV-B radiation and the time for the release of HaCaT-Exos. Furthermore, the Schwann cells were injured through the HG model, isolated by ultracentrifugation from the HaCaT cell culture supernatant were co-incubated within the injured cells. It was found that UV-B treated HaCaT-Exos decrease apoptosis and increase the viability of injured SC by transferring miR-222 from HaCaT cells to them via exosomes, hence protecting them.
Page Count
64
Department or Program
Department of Pharmacology and Toxicology
Year Degree Awarded
2020
Copyright
Copyright 2020, all rights reserved. My ETD will be available under the "Fair Use" terms of copyright law.
