Publication Date
2021
Document Type
Thesis
Committee Members
Ioana Pavel, Ph.D. (Advisor); David Dolson, Ph.D. (Committee Member); Steven Higgins, Ph.D. (Committee Member)
Degree Name
Master of Science (MS)
Abstract
Nanomaterials have attracted significant attention in the last decade, with applications in everyday products. Amongst all known nanomaterials in use, silver is the leading metal, present in 531 different types of products, owing to their unique optical, electrical, antimicrobial, and thermal properties. Though silver nanoparticles (AgNPs) come in contact regularly with the general population, there is little known about their toxicity mechanism due to limited techniques for thorough analysis. CytoViva Hyperspectral Imaging (HSI) shows potential for filling these gaps. In this study, borohydride-capped AgNPs with an approximate diameter of 10 nm were synthesized using the modified Creighton method and characterized by Raman spectroscopy, inductively coupled plasma optical emission spectroscopy (ICP-OES), ultraviolet-visible absorption spectroscopy (UV-Vis), and CytoViva HSI. Vero 76 cells were then incubated for 2 hr with 0.0, 0.1, and 3.0 mg L-1 Ag+ and 3.0 mg L-1 borohydride-capped AgNPs and compared to control Vero 76 cells via CytoViva HSI. The hyperspectral signatures were deconvoluted utilizing peak centers of cholesterol, cytochrome c, and phospholipids determined though a Voigt fit. Visually, the 3.0 mg L-1 Ag+ and AgNP exposures displayed cell degradation through the presence of cell debris and change in cell shape. Additionally, t-tests performed on the deconvoluted peaks showed statistical significance in Ag+ and AgNP groups, especially with the peak associated with cytochrome c, indicating mitochondrial interaction.
Page Count
59
Department or Program
Department of Chemistry
Year Degree Awarded
2021
Copyright
Copyright 2021, all rights reserved. My ETD will be available under the "Fair Use" terms of copyright law.
