Publication Date
2022
Document Type
Thesis
Committee Members
Adrian Corbett, Ph.D. (Advisor); Christopher Wyatt, Ph.D. (Committee Member); Keiichiro Susuki, M.D., Ph.D. (Committee Member)
Degree Name
Master of Science (MS)
Abstract
Multiple sclerosis (MS) affects an estimated one million people in the US alone (Wallin et al., 2019). We modeled MS in rats using lysolecithin injection into the corpus callosum to diminish motor function unilaterally through demyelination, meanwhile treating the injury with Fluoxetine, Ibuprofen, and ascorbic acid (FIAA) to increase neurogenesis and oligodendrogenesis. Motor function was assessed using the Montoya Staircase test pre- and post-surgery. Motor capabilities recovered in the contralateral limb, but not in the ipsilateral, and recovery was not significantly affected by treatment. We identified microglia by CX3CR1 and examined its distribution in the adult neurogenic niche, the SVZ of the lateral ventricles. Microglia displayed significant regional differences with posterior ventricle slices containing more microglial area than those of the anterior, and the treatment had no significant effect on this distribution. We found CX3CR1 area to be more negatively correlated with functional recovery in treated animals compared to controls, indicating that the treatment may favor recovery in animals with a greater proportion of activated microglia.
Page Count
88
Department or Program
Department of Neuroscience, Cell Biology, and Physiology
Year Degree Awarded
2022
Copyright
Copyright 2022, some rights reserved. My ETD may be copied and distributed only for non-commercial purposes and may not be modified. All use must give me credit as the original author.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
