Publication Date
2023
Document Type
Thesis
Committee Members
Adrian Corbett, Ph.D. (Committee Chair); Michael Matott, Ph.D. (Committee Member); Christopher Wyatt, Ph.D. (Committee Member)
Degree Name
Master of Science (MS)
Abstract
Neurodegenerative diseases such as multiple sclerosis affect millions of people around the globe, and promising treatments to counter its progress have been studied extensively. In our study, we used Dawley rats and tested them using the Montoya Staircase test to evaluate their ability to use their forelimbs. After training, surgery was performed using lysolecithin to induce focal demyelination on the rats. After the surgery a drug combination of fluoxetine, ibuprofen, and ascorbic acid was used on injured rats and they were then retested on the Montoya Staircase test. They were then sacrificed and were stained for doublecortin (DCX) and nuclear factor kappa B (NFkB) to see how the drug combination would affect neurogenesis and remyelination. Our results found that when introduced, the drug combination caused a decrease in the DCX and an increase in the NFkB staining. This suggests that the drug combination would have an impact on the differentiation of oligodendrocytes during recovery since a decrease in DCX would indicate that the DCX is assisting with the proliferation and migration of new oligodendrocytes while an increase in NFkB would infer that as NFkB is being activated more oligodendrocyte progenitor cells are being led to survival.
Page Count
65
Department or Program
Department of Neuroscience, Cell Biology, and Physiology
Year Degree Awarded
2023
Copyright
Copyright 2023, some rights reserved. My ETD may be copied and distributed only for non-commercial purposes and may not be modified. All use must give me credit as the original author.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
ORCID ID
0009-0001-0823-5360
