Publication Date

2024

Document Type

Thesis

Committee Members

Sherif Elbasiouny, Ph.D. (Advisor); Jaime Ramirez-Vick, Ph.D. (Committee Member); Keiichiro Susuki, M.D., Ph.D. (Committee Member)

Degree Name

Master of Science in Biomedical Engineering (MSBME)

Abstract

This research explores how motor neurons (MNs) and Kv2.1 clustering change with age, emphasizing sex differences and MN subtypes. We found that MN density decreases with age in both sexes, while soma size increases in male mice. FF MNs were the most affected, and old weak mice had smaller MNs than their stronger counterparts, underscoring FF MN vulnerability. Baseline studies revealed that FF and FI MNs have larger Kv2.1 clusters compared to FR and S MNs. Female mice had smaller, denser Kv2.1 clusters than male mice, suggesting less clustering in females. With age, Kv2.1 clusters grew larger but became less dense and intense, indicating increased clustering. Old weak mice showed even more pronounced clustering than strong ones, linking Kv2.1 changes to age-related weakness. These findings highlight the susceptibility of FF MNs to aging and position Kv2.1 clustering as a key factor in motor neuron function and age-related decline.

Page Count

85

Department or Program

Department of Biomedical, Industrial & Human Factors Engineering

Year Degree Awarded

2024


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