Publication Date
2020
Document Type
Thesis
Committee Members
Katherine J. Excoffon, Ph.D. (Advisor); Abimbola O. Kolawole, Ph.D. (Advisor); Quan Zhong, Ph.D. (Committee Member)
Degree Name
Master of Science (MS)
Abstract
Adenovirus infection mostly triggers cold-like symptoms, but in some cases, adenovirus infection may lead to severe symptoms and death. The primary receptor for many adenoviruses is the Coxsackievirus and Adenovirus receptor (CAR). There are two transmembrane isoforms of CAR; CAR^Ex7 and CAR^Ex8. CAR^Ex7 localizes at the basolateral surface and CAR^Ex8 localizes at the apical surface. I hypothesized that knockout of CAR^Ex8 will prevent apical adenovirus transduction, while knockout of all CAR isoforms will prevent apical and basolateral adenovirus transduction. To test this, I utilized CRISPR/Cas9 to knockout endogenous CAR^Ex8 or all CAR isoforms in MDCK-CAR^Ex8 cells. The results indicate that knockdown of CAR^Ex8 significantly decreased apical adenovirus transduction. Moreover, the knockdown of total CAR resulted in a decline in all adenovirus transduction. The data indicate that CAR^Ex8 localization promotes adenovirus entry, which might provide potential targets for adenovirus gene therapy.
Page Count
76
Department or Program
Department of Biological Sciences
Year Degree Awarded
2020
Copyright
Copyright 2020, all rights reserved. My ETD will be available under the "Fair Use" terms of copyright law.
