Publication Date
2010
Document Type
Thesis
Committee Members
David Cool (Committee Member), Andrea Hoffmann (Advisor), Osvaldo Lopez (Committee Member)
Degree Name
Master of Science (MS)
Abstract
This study determined the tissue-specific effect of adipose stem cells (ASC) within a melanoma environment for development of a cell-based melanoma therapy. Analysis included a subcutaneous B16-F1 melanoma model using thirty-one C57BL/6 wild-type mice. Melanoma xenografts were treated with cell-based therapies of CFDA-SE-labeled human fibroblasts HF20x (control), non-differentiated ndASC or hematopoietic-differentiated HdASC. No tumor regression was observed in presence of cell-based therapies, thus, the HdASC group demonstrated an increase in tumor growth accompanied with an up-regulated macrophage response, and increased angiogenesis. In addition, this group demonstrated a decrease in Melan-A tumor marker and interferon-γ expression suggesting that ASC-supported tumor angiogenesis and macrophage immune response are accompanied with an inflammatory water influx and increased tumor porosity with no effect on tumor cell proliferation. In addition, histology demonstrated CFDA-SE-labeled HF20x, ndASC, or HdASC inside tumors with no signs of cell death or apoptosis suggesting an immuno-suppressive effect of human ASC on the mouse immune system.
Page Count
98
Department or Program
Department of Pharmacology and Toxicology
Year Degree Awarded
2010
Copyright
Copyright 2010, all rights reserved. This open access ETD is published by Wright State University and OhioLINK.
