Cell Volume and Metabolic Dependence of NEM-Activated K+-Cl- Flux in Human Red Blood Cells
Document Type
Article
Publication Date
1985
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Abstract
The effects of incubation in anisosmotic media and of metabolic depletion on ouabain-resistant (OR) Cl--dependent K+ influxes stimulated by N-ethylmaleimide (NEM) were studied in human red blood cells using Rb+ as K+ analogue. The NEM-stimulated but not the basal Rb+-Cl- influx measured in phosphate-buffered anisosmotic media was found to be cell volume dependent. When cellular ATP, [ATP]c, was lowered to less than 0.10 of its initial level by exposure to nonmetabolizable 2-deoxy-D-glucose, the NEM-stimulated but not the basal Cl--dependent Rb+ influxes were abolished. Metabolically depleted red blood cells subsequently repleted by incubation in glucose plus inosine regained the NEM-inducible Rb+ (K+) transport activity. The difference in the time course of ATP breakdown and Rb+ influx inhibition suggests that energization of the NEM-stimulated Rb+ flux by metabolism may involve factors additional to ATP.
Repository Citation
Lauf, P. K.,
Perkins, C. M.,
& Adragna, N. C.
(1985). Cell Volume and Metabolic Dependence of NEM-Activated K+-Cl- Flux in Human Red Blood Cells. American Journal of Physiology - Cell Physiology, 18 (1), C124-C128.
https://corescholar.libraries.wright.edu/ptox/260
DOI
10.1152/ajpcell.1985.249.1.c124
